• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Support for in vitro cultures and its manufacturing procedure, the procedure is as follows: To. Heat casting of polypropylene pellets B. Obtaining continuous fibers of thickness C. Mechanical treatment of polypropylene continuous fibers D. Cut to a predetermined length of the polypropylene fibers obtained in the previous stage And. Packed in bales of the fibers obtained in step d) F. Unpacking of the bullets obtained in stage e) G. Separated from the fibers obtaining support for in vitro cultures. The support for in vitro cultures obtained is 100% polypropylene, so its chemical composition is always the same and the comparison between tests is not affected by the chemical composition of the support as in the case of existing supports, in addition said support it is reusable and sterilizable by traditional methods. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2650628A1
    申请号:ES201630980
    申请日:2016-07-18
    公开日:2018-01-19
    发明作者:Enrique FIGUEROA YAÑEZ
    申请人:Geotexan S A；Geotexan SA；
    IPC主号:
    专利说明:

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    DESCRIPTION
    Support for in vitro cultures and their manufacturing process Object of the invention
    The object of the present invention is a novel process for obtaining polypropylene fibers obtained from geotextile fabrics intended to be the support for the in vitro culture of plant tissues.
    Background of the invention
    In vitro culture of plants is a set of techniques that have in common the fact that an explant, that is a part of plant tissue, is aseptically grown in an artificial medium of defined chemical composition and incubated under controlled environmental conditions.
    These techniques can be used in vegetables as tools for micropropagation, rapid propagation of clones, virus and disease elimination, haploid production, microplast isolation and utilization, embryo culture, phytochemical production, genetic engineering, mutation and cell selection, production of synthetic seeds and basic studies of anatomy, development, physiology and cellular nutrition.
    In some of the aforementioned techniques, plant tissues may need to be supported or placed on or inside a solidified aqueous gel usually with agar or in a liquid medium.
    Agar is a gelatinous substance that is obtained from the algae cell wall of the geledium, euchema and gracilaria genera, which when dissolved in hot water and subsequently cooled becomes gelatinous, its neutral charge and its low degree of chemical complexity make Unlikely to interact with biomolecules such as protein and nucleic acids, so it is especially used as a support for in vitro cultures of plant tissues.
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    However, the composition of the agar cannot be 100% controlled, making it difficult to compare research experiments.
    The present invention advocates a method for obtaining a novel support for in vitro cultures of plants whose chemical composition can be fully controlled and which is also reusable and easily sterilizable with traditional methods.
    Description of the invention
    The method of manufacturing a support for in vitro cultures comprises the following steps:
    a) heat smelting of polypropylene pellets
    b) obtaining continuous fibers of predetermined thickness by passing the polypropylene melt through pressurized air through rows.
    c) mechanical treatment of continuous polypropylene fibers
    d) cut to a predetermined length of the polypropylene fibers obtained in the previous step
    e) packed in bullets of the fibers obtained in step d)
    f) unpacking the bullets obtained in stage e)
    g) separated and carded from the fibers obtaining support for in vitro cultures.
    The support for in vitro cultures obtained by the procedure described above is 100% polypropylene. Since its chemical composition is always the same, the comparison between tests is not affected by the chemical composition of the support, as is the case when using the existing supports on the market, so that comparison between the tests is consistent, in addition said Support is reusable and sterilizable by traditional and well known methods.
    Preferred embodiment
    A preferred embodiment of the method of manufacturing a support for in vitro cultures, object of the present invention is described below and has the following steps:
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    a) heat smelting of the polypropylene pellet, preferably the melting of the pellet is carried out by conduits heated by thermal oil.
    b) obtaining continuous fibers of predetermined thickness by passing the polypropylene melt through pressurized air through rows of predetermined diameter.
    c) mechanical treatment of continuous polypropylene fibers to achieve the desired mechanical characteristics.
    d) cut to a predetermined length of the polypropylene fibers obtained in the previous step
    e) packed in bullets of the fibers obtained in step d)
    f) unpacking the bullets obtained in stage e)
    g) separated and carded from the fibers obtaining support for in vitro cultures.
    权利要求:
    Claims (2)
    [1]

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    1. Method of manufacturing a support for in vitro cultures characterized in that it comprises the following stages:
    to. heat smelting of polypropylene pellets
    b. obtaining continuous fibers of predetermined thickness by passing the polypropylene melt through pressurized air through rows.
    C. mechanical treatment of continuous polypropylene fibers
    d. cut to a predetermined length of the polypropylene fibers obtained in the previous step
    and. packed in bullets of the fibers obtained in step d)
    F. unpacking the bullets obtained in stage e)
    g. separated from the fibers obtaining support for in vitro cultures.
    [2]
    2. Support for in vitro cultures characterized in that it is made of polypropylene and is manufactured according to the method of the preceding claim.
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    同族专利:
    公开号 | 公开日
    ES2650628B1|2018-09-06|
    引用文献:
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    法律状态:
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    优先权:
    申请号 | 申请日 | 专利标题
    ES201630980A|ES2650628B1|2016-07-18|2016-07-18|SUPPORT FOR IN VITRO CROPS AND ITS MANUFACTURING PROCEDURE|ES201630980A| ES2650628B1|2016-07-18|2016-07-18|SUPPORT FOR IN VITRO CROPS AND ITS MANUFACTURING PROCEDURE|
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